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Image Search Results
Journal: Cancer Immunology, Immunotherapy : CII
Article Title: A new tyrosinase epitope recognized in the HLA-B*4002 context by CTL from melanoma patients
doi: 10.1007/s00262-008-0551-0
Figure Lengend Snippet: a Identification of the cDNA regions coding for the B*4002 restricted tyrosinase epitope. Tyrosinase fragments were obtained by exonuclease digestion. Fragments recognized by the specific M88-74 CTL clone are indicated with a positive sign in the right column. The positions are indicated in base pairs. Fragments were co-transfected into COS-7 cells with the appropriate HLA cDNA. Fourty-eight hours after transfection, cells were incubated for 6 h at 37°C with the CTL clone and TNF secreted in the supernatant was measured by assessing its cytotoxicity for WEHI 164 clone 13. b TNF secretion by M88-74 CTL clone in response to COS-7 cells transfected with the B*4002 cDNA and the full-length cDNA coding for tyrosinase and with the tyrosinase fragments 1–933 and 1–975
Article Snippet: MMP-1 MMP-2 MMP-3 37 38 39 40 41 42 43 44 45 46 MMP-7 MMP-9 MMP-14 MAGE-D Eph 2 Her-2neu RAS WT RAS mut Hsp70 Steap Open in a separate window The cDNA coding for
Techniques: Transfection, Incubation